Extracellular matrix substrates produced by cells cultured in 20% O 2, combined with an environment of pH 7.0, provided the least favorable conditions for binding of strain 19660.
Binding in the presence of antibodies directed against laminin-5 was also measured. The mean number of adherent bacteria per counted per field ± SEM ( n = 15) was determined for combinations of bacteria, extracellular matrix substrate, pH, and O 2. aeruginosa strains ATCC 19660 or PAO1 (suspended in pH 7.0 or 7.5 buffer) were cultured on extracellular matrix substrates in 2% or 20% O 2.
Extracellular matrix substrates were prepared from human corneal epithelial cells cultured in 2% or 20% O 2. To measure the effect of extracellular matrix substrate, pH, and O 2 on Pseudomonas aeruginosa binding.
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